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. 2020 Dec 17;11:6409. doi: 10.1038/s41467-020-20262-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Confocal imaging of modified HeLa cell line expressing homozygous sfGFP-tagged POLR1C gene. Endogenous GFP signal, representing Pol I and III (green), DAPI staining (magenta) and overlay of both channels are shown. Scale bar: 5 µm. Shown is a representative image of four independent experiments. b Affinity-purified human RNA Pol III from HeLa nuclear and cytoplasmic fractions. Confirmed RNA Pol subunits are labelled. Shown is a representative image of two separate experiments. c Purified human RNA Pol III (hPol III) from large-scale whole-cell lysate with Pol III subunits marked. Shown is an excised lane of a representative image of three separate purifications. d RNA extension assay of fluorescently labelled FAM-15mer RNA primer by purified human Pol III. Marked is Pol III-mediated primer extension. The molecular marker displays expected sizes for 15 and 21 nucleotides (nt). Displayed is a representative image of seven independent repeats. Source data are provided as a Source Data file.