Skip to main content
. 2020 Dec 17;11:6409. doi: 10.1038/s41467-020-20262-5

Fig. 5. Cycloheximide chase assay investigation of RPC5 protein stability.

Fig. 5

HEK293T cells were seeded, endogenous RPC5 was knocked down via siRNA and either the FL a, ΔtWHD2 b, or ΔC c, RPC5 constructs were transfected. After 24 h, cycloheximide was added in at a concentration of 300 µg/ml and cells lysed at the specified time points. RPC1, 2, 4 and 5 (as shown via HA-tag antibody) levels were probed by western blotting. Displayed images are representative of three independent experiments. Source data are provided as a Source Data file.