. 2020 Dec 4;12(1):1847628. doi: 10.1080/19490976.2020.1847628

Table 1.

Multivariate factor analyses

Figure	Analysis	Y-variable	X–variables	VIP-value
Figure 1a	OPLS^a	IL-13	NGS detected bacteria^d	>1.5
Figure 1b	OPLS^a	IL-5	NGS detected bacteria^d	>1.5
Figure 1c	OPLS^a	IL-6	NGS detected bacteria^d	>1.5
Figure 1d	OPLS^a	TNF	NGS detected bacteria^d	>1.5
Figure 1e	OPLS^a	IL-1β	NGS detected bacteria^d	>1.5
Figure 2a	OPLS^a	IL-13	Culture detected bacteria^e	>1.2
Figure 2b	OPLS^a	IL-5	Culture detected bacteria^e	>1.2
Figure 2c	OPLS^a	IL-6	Culture detected bacteria^e	>1.2
Figure 2d	OPLS^a	TNF	Culture detected bacteria^e	>1.2
Figure 2e	OPLS^a	IL-1β	Culture detected bacteria^e	>1.2
Figure 4a	OPLS^a	CD45RO	NGS detected bacteria^d	>1.5
Figure 4b	OPLS^a	CD45RO	Culture detected bacteria^e	>1.2
Figure 5a	PCA^b		Cytokine responses^c Environmental factors^f
Supplemental Figure 1a	OPLS^a	CD45RO	Cytokine responses^c
Supplemental Figure 5a	OPLS^a	IFN-γ	NGS detected bacteria^d	>1.5
Supplemental Figure 5b	OPLS^a	IFN-γ	Culture detected bacteria^e	>1.2
Supplemental Figure 6A	OPLS^a	Ceasaran section	Cytokine responses^c CD45RO
Supplemental Figure 6B	OPLS^a	Female	Cytokine responses^c CD45RO
Supplemental Figure 6C	OPLS^a	Antibiotics delivery	Cytokine responses^c CD45RO
Supplemental Figure 6D	OPLS^a	Formula fed	Cytokine responses^c CD45RO
Supplemental Figure 6E	OPLS^a	Breast fed exclusively	Cytokine responses^c CD45RO
Supplemental Figure 6F	OPLS^a	Breast fed partially	Cytokine responses^c CD45RO
Supplemental Figure 6G	OPLS^a	Farm	Cytokine responses^c CD45RO
Supplemental Figure 6H	OPLS^a	Un-pasteurized milk	Cytokine responses^c CD45RO
Supplemental Figure 6I	OPLS^a	Cat	Cytokine responses^c CD45RO
Supplemental Figure 6J	OPLS^a	Dog	Cytokine responses^c CD45RO

^aOPLS = Orthogonal projection to latent structures by means of partial least squares

^bPCA = Principal Component Analysis

^cCytokine responses include: IL-13, IL-5, IL-6, IL-1β, TNF and IFN-γ

^dBacteria detected by NGS that include Actinomycetaceae, Bifidobacteriaceae, Corynebacteriaceae, Micrococcaceae, Propionacteriaceae, Atopobiaceae, Coriobacteriaceae, Eggerthellaceae, Bacteroidaceae, Barnesiellaceae, Prevotellaceae, Rikenellaceae, Tannerellaceae, Bacillales Family XI, Staphylococcacea, Carnobacteriaceae, Enterococcaceae, Lactobacillaceae, Streptococcaceae, Clostridiaceae, Family XI, Family XIII, Lachnospiraceae, Peptostreptococcaceae, Ruminococcaceae, Erysipelotrichaceae, Acidaminococcaceae, Veillonellaceae, Desulfovibrionaceae, Burkholderiaceae, Neisseriaceae, Akkermansiaceae, non-E. coli Enterobacteriaceae, Escherichia, and Pasteurellaceae

^eBacteria detected by culture techniques that include Enterococcus spp, enterobacterium non- Ecoli, Clostridium spp, Clostridium difficilie, Lactobacillus spp, Staphylococcus aureus, E.coli, Bifidobacterium spp. Yeast and ratio of anaerobic/aerobic data are also included in these analysis.

^fEnvironmental factors that include being born by Cesarian section, sex, antibiotics at delivery (mother), having older siblings, children who were breast fed or formula fed partially or exclusively, living on a farm, intake of un-pasteurized milk or farm milk and having cats or dogs.