Figure 4.

CRISPR-Mediated Base Editing Using ABEs.

(A) ABEs are composed of nCas9 (D10A) fused to an adenine deaminase catalytic domain (ecTadA-ecTadA∗) that mediates adenine deamination in the so-called editing window at the 5′ end of the non-targeted sequence.

(B) After A deamination into I (inosine), nicking of the non-edited strand and intervention of DNA repair/replication mechanisms produce A-to-G conversions, with very low rates of by-products.

The schemes are not at scale and are for illustrative purposes only.