Figure 5.

Conversion between AtTPS18 and Its Close Homologs.

C7 ((+)-thalianatriene), C8 ((−)-retigeranin), and C9 ((−)-caprutriene) are the main products of AtTPS18, AtTPS19, and Cru237, respectively.

(A) Comparison of the models of AtTPS18 (blue) and AtTPS19 (orange). The different amino acids in the catalytic pockets of AtTPS18 and AtTPS19 are highlighted.

(B) GC–MS (SIM mode, m/z 340 for C₂₅H₄₀) analysis of AtTPS18, AtTPS19, and their mutants. The peaks of C7 (the main product of AtTPS18) and C8 (the main product of AtTPS19) are marked with arrows.

(C) Comparison of the models of AtTPS18 (blue) and Cru237 (orange). The amino acid at position 353 is highlighted.

(D) GC–MS (SIM mode, m/z 340 for C₂₅H₄₀) analysis of AtTPS18, Cru237, and their mutants.