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. 2019 Nov 9;1(2):100007. doi: 10.1016/j.xplc.2019.100007

Figure 5.

Figure 5

FR Light Responses Mediated by phyA-NLS-YFP Depend on HFR1 and HY5.

(A) HY5 and HFR1 are required for phyA-NLS-YFP–mediated inhibition of hypocotyl growth. Seedlings were grown in the dark or in FR light of different intensities. After 5 days, hypocotyl length was measured. Bars show mean hypocotyl length of ≥20 seedlings ± SD. Replicates are shown in Supplemental Figure 5.

(B) Enhanced anthocyanin accumulation in phyA-NLS-YFP expressing seedlings depends on HY5 and HFR1. Seedlings were grown in the dark or in continuous FR light (13 μmol m−2 s−1) for 5 days. Then, anthocyanin was extracted and relative amounts were quantified. Bars show mean values of A535–A650 per seedling of three replicates ± SD.

(C) Subcellular localization of phyA-NLS-YFP in hfr1-4 and hy5-215. Four-day-old dark-grown seedlings were used for fluorescence microscopy. The seedlings were analyzed directly (D), after 6 h irradiation with FR light (13 μmol m−2 s−1), or after 6 h FR light exposure followed by 5 min of R light (8 μmol m−2 s−1). Scale bar represents 5 μm.