Figure 6.

Differences between Wild Type and phyA-NLS-YFP Expressing Seedlings.

(A) PhyA-NLS-YFP expressing seedlings are hypersensitive to R and FR light. Seedlings were grown for 4 days in R light (left), FR light (right), or in the dark. Data show the mean hypocotyl length of ≥18 seedlings relative to dark-grown (D) seedlings ±SE. Absolute values are shown in Supplemental Figure 6.

(B) Seedlings expressing high levels of phyA-NLS-YFP are constitutively photomorphogenic. Col-0 and three independent transgenic lines expressing phyA-NLS-YFP in phyA-211 background (#7129, #7137, and #7146) were grown for 4 days in the dark. Germination was induced by different light treatments: (1) 5 min R light, 2 h 55 min dark, 5 min RG9 (long-wavelength FR light); (2) 3 h R light, 5 min RG9; (3) 3 h R light.

(C) PhyA protein levels in phyA-NLS-YFP and phyA-YFP expressing lines. Seedlings were grown for 4 days in the dark. Total protein was extracted, separated on an SDS–PAGE, and blotted onto PVDF membrane. A phyA-specific antibody was used to detect phyA; actin was detected as loading control.

(B and C) Line #7129 was used for experiments in Figures 3, 5, and 6.