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. 2020 Feb 19;1(2):100033. doi: 10.1016/j.xplc.2020.100033

Figure 1.

Figure 1

Nup160 Regulates Flowering Time in Arabidopsis.

(A) Schematic diagram shows the gene structure of Nup160, the location of T-DNA insertion sites in nup160-2 (SALK_016091) and nup160-5 (SALK_133728), and the target site of the AmiR in 35S:AmiR-nup160. Exons are represented by black boxes, while introns and untranslated regions are indicated by black lines.

(B)Nup160 expression is undetectable in nup160-2 and nup160-5 by semi-quantitative PCR using primers that amplify the 5′ end of the Nup160 transcript. TUB2 was amplified as an internal control.

(C)nup160-2 and nup160-5 exhibit early flowering under both long days and short days.

(D) Flowering time of nup160 mutants under long days and short days (n ≥ 16, ±SD).

(E) Distribution of flowering time in T1 transgenic plants harboring the Nup160 genomic fragment in nup160-2 background grown under long days.

(F) Downregulation of Nup160 in independent 35S:AmiR-nup160 transgenic plants correlates to the degree of early flowering (left axis). Expression levels of Nup160 determined by quantitative real-time PCR in 9-day-old seedlings were normalized against the expression of TUB2 and shown as relative values (red line) to the wild-type level as 1 (right axis). Error bars indicate SD.