Figure 2.

Nup160 Represses FT Expression in the Morning through CO.

(A and B) Diurnal expression of FT(A) and CO(B) determined by quantitative real-time PCR in 9-day-old wild-type (WT) and nup160-2 seedlings grown under long days. Samples were harvested every 4 hours from the onset of illumination, which are shown in hours as Zeitgeber time (ZT). Gene expression levels were normalized against the expression of TUB2 and shown as relative values to the highest level in each panel as 1.0. Asterisks indicate statistically significant differences in FT expression between WT and nup160-2 plants (two-tailed paired Student's t-test, P < 0.005).

(C and D) Expression levels of FT at ZT4 (C) and ZT16 (D) determined by quantitative real-time PCR in 9-day-old WT, nup160-2, co-9, and co-9 nup160-2 seedlings grown under long days. Gene expression levels were normalized against the expression of TUB2 and shown as relative values to the WT level as 1.0. Error bars indicate SD.

(E) Flowering time of various mutants grown under long days (n ≥ 16, ±SD).