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. 2020 Feb 19;1(2):100033. doi: 10.1016/j.xplc.2020.100033

Figure 3.

Figure 3

Nup160 Destabilizes CO Protein during the Morning.

(A) Flowering time of WT, nup160-2, co-9, SUC2:3FLAG-CO, SUC2:3FLAG-CO nup160-2, and SUC2:3FLAG-CO co-9 plants grown under long days (n ≥ 16, ±SD). Asterisks indicate a statistically significant difference between specified genotypes (two-tailed paired Student's t-test, P < 0.005).

(B and C) Diurnal expression of FT(B) and CO(C) mRNA determined by real-time qPCR in 9-day-old SUC2:3FLAG-CO and SUC2:3FLAG-CO nup160-2 seedlings grown under long days. Gene expression levels were normalized against the expression of TUB2 and shown as relative values to the highest level in each panel as 1. Error bars indicate SD. Asterisks indicate statistically significant differences in FT expression between two genotypes (two-tailed paired Student's t-test, P < 0.005).

(D) CO protein expression in 9-day-old SUC2:3FLAG-CO and SUC2:3FLAG-CO nup160-2 seedlings grown under long days. Nuclear protein extracts from seedlings collected at different ZT time points were detected by anti-FLAG antibody. Histone H3 is shown as a loading control.

(E) Quantification of CO protein abundance against the H3 expression levels in (D).