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. 2020 Feb 19;1(2):100033. doi: 10.1016/j.xplc.2020.100033

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© 2020 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Nup160 Is Required for the Nuclear Envelope Localization of HOS1.

(A and C) Subcellular localization of GFP-HOS1 in meristematic root cells of 5-day-old GFP-gHOS1 hos1-3 seedlings in either WT (A) or nup160-2(C) background. The right panels show the magnified views of the boxes indicated in (A) and (C), respectively. Scale bars correspond to 10 μm.

(B and D) Measurement of fluorescence intensity profiles of GFP-HOS1 along the lines indicated in the enlarged figures shown in (A) and (C), respectively. Similar results were observed in meristematic root cells of 20 independent plants for each genotype.

(E and G) Subcellular localization of GFP-HOS1 in leaf phloem companion cells of 9-day-old SUC2:nlsRFP GFP-gHOS1 hos1-3 seedlings in either WT (E) or nup160-2 background (G). GFP, GFP fluorescence; RFP, RFP fluorescence; Merge, merge of GFP and RFP images. Scale bars correspond to 10 μm.

(F and H) Measurement of fluorescence intensity profiles of GFP-HOS1 and nlsRFP along the lines indicated in (E) and (G), respectively. The similar results were observed in leaf phloem companion cells of 10 independent plants for each genotype. Scale bars correspond to 10 μm (A, C, E, and G).