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. 2020 Feb 19;1(2):100033. doi: 10.1016/j.xplc.2020.100033

Figure 6.

Figure 6

The Nup160-HOS1 Module Prevents Pre-activation of FT through Destabilizing CO Protein.

(A) Effects of nup160-2 and hos1-3 on CO protein abundance in SUC2:3FLAG-CO. 9-day-old seedlings of SUC2:3FLAG-CO in the WT, nup160-2, hos1-3, and nup160-2 hos1-3 backgrounds were collected at different ZT time points under long days. Nuclear protein was extracted and detected with anti-FLAG antibody. Histone H3 is shown as a loading control.

(B) Quantification of CO protein abundance against the H3 expression levels in (A).

(C) Diurnal expression of FT mRNA determined by real-time qPCR in 9-day-old WT, nup160-2, hos1-3, and nup160-2 hos1-3 seedlings grown under long days. Samples were harvested every 4 hours from ZT0, except ZT20. Gene expression levels were normalized against the expression of TUB2 and shown as relative values to the WT level as 1.0. Error bars indicates SD. Asterisks indicate statistically significant differences in FT expression between WT and nup160-2, hos1-3, or nup160-2 hos1-3 (two-tailed paired Student's t-test, P < 0.005).

(D) Flowering time of nup160-2, hos1-3, nup160-2 hos1-3, and their crossed progenies with SUC2:3FLAG-CO grown under long days (n ≥ 16, ±SD). n.s. indicates no significant difference (two-tailed paired Student's t-test, P > 0.05).