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Effect of EA on SIRT1 expression in the skeletal muscle of HFD-fed IR rats. SIRT1 levels were decreased significantly in HFD-fed IR rats compared with those in the NC group. EA increased SIRT1 expression in HFD-fed IR rats. Total protein was obtained from the quadriceps muscles of IR rats and subjected to western blot for SIRT1. β-tubulin was used as the loading control. Data are presented as the mean ± SD of eight rats per group. *P < 0.05 vs. NC; #P < 0.01 vs. HFD.
