Fig 2. JD1 is bacteriostatic and bactericidal under conditions that compromise the outer membrane barrier.

A, B) Dose response curves monitoring bacterial growth under the indicated conditions/strains normalized to DMSO for A) S. Typhimurium and B) E. coli K12. Data are normalized to growth in DMSO (100%). Mean and SEM of at least three independent biological replicates performed with technical triplicates. C-J) Log phase cultures of the indicated strains/conditions were treated at time 0 with either DMSO or the corresponding MIC₉₅ concentration of JD1 (Table 1). (C-F) Cultures were monitored for OD₆₀₀. The red dotted line denotes the limit of detection. (G-J) Cultures were also plated for enumeration of CFU. Mean and SEM of three biological replicates performed with technical triplicates. The medium used was LB unless otherwise indicated next to the strain name.