Table 1. Concentrations of JD1 that inhibit bacteria under different conditions.
| Species (strain) | Genotype | Growth conditions (read-out) | IC95 | |
|---|---|---|---|---|
| μM | μg/mL | |||
| S. Typhimurium (SL1344) | WT | In macrophages (GFP+ Macrophage Area) | 1.2 | 0.49 |
| WT | In macrophages (CFU enumeration) | 1.3 | 0.53 | |
| WT | In HeLas (GFP+ Macrophage Area) | 5.5 | 2.24 | |
| WT | In HeLas (CFU enumeration) | 7.1 | 2.89 | |
| Species (strain) | Genotype | Growth conditions (OD600) | MIC951 | |
| μM | μg/mL | |||
| S. Typhimurium: The strain background is SL1344 (WT) unless otherwise indicated | WT | LB | >150 | >61.0 |
| 14028 | LB | >150 | >61.0 | |
| WT | LB, 0.5 μg/mL PMB | 14.1 | 5.7 | |
| 14028 | LB, 0.5 μg/mL PMB | 15.9 | 6.5 | |
| WT | LB, 10 μg/mL PMBN | 137.8 | 56.3 | |
| WT | M9, 1 μM Mg2+ | 77.2 | 31.5 | |
| WT | LPM | 88.9 | 36.3 | |
| ΔacrAB | LB | 31.9 | 13.0 | |
| E. coli (K12) | WT | LB | >150 | >61.0 |
| lptD4213 | LB | 25.9 | 16.0 | |
| ΔtolC | LB | 20.1 | 8.2 | |
1 1X MIC is defined as MIC95