Table 2. Nile red accumulation and loss in JD1-resistant mutants.
| Nile red accumulation at Time 0 (no glucose) |
Slope of Nile red loss after CCCP removal1 | ||||
|---|---|---|---|---|---|
| Strain | - CCCP2 | + CCCP | Ratio, +/- CCCP | - glucose | + glucose |
| Wild type | 1.00 | 2.27 | 2.3 | 1 | 8.0 |
| Mar1 | 0.52 | 2.09 | 4.0 | 1.1 | 9.4 |
| Mutant 6 | 0.51 | 4.76 | 9.3 | 2.0 | 23.6 |
| Mutant 5 | 0.47 | 2.24 | 4.8 | 1.5 | 11.4 |
| Mutant 3 | 0.39 | 4.82 | 12.3 | 2.7 | 24.8 |
| Mutant 2 | 0.37 | 4.35 | 11.9 | 3.1 | 21.4 |
| Mutant 4 | 0.25 | 1.76 | 7.1 | 1.3 | 9.1 |
| Mutant 1 | 0.04 | 0.25 | 7.0 | 0.24 | 0.8 |
1 Slope was calculated for the linear range of the experiment (the first 30 minutes) and normalized to wild type without glucose.
2 Mutants are arranged from highest to lowest Nile red retention in the absence of CCCP.