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. Author manuscript; available in PMC: 2020 Dec 18.
Published in final edited form as: J Pathol. 2020 May 18;251(3):284–296. doi: 10.1002/path.5451

Figure 3.

Figure 3.

FDXR is required for p73 expression. (A) HCT116 and (B) RKO cells were transfected with scrambled or FDXR siRNA for 3 days, followed with or without doxorubicin treatment for 18 h. The levels of FDXR, TAp73 and β-actin proteins were examined by western blotting. (C) SW480 cells were transfected with a scrambled or FDXR siRNA for 3 days and the levels of FDXR, TAp73 and actin were determined by western blotting. (D) Isogenic control and FDXR+/−/− HCT116 cells were mock-treated or treated with camptothecin or doxorubicin for 18 h, and the levels of FDXR, TAp73 and β-actin proteins were determined by western blotting. (E) The levels of FDX1, TAp73 and β-actin were measured in isogenic control and FDX1-KO HCT116 cells. (F,G) HCT116 and TP53−/− HCT116 cells were transiently transfected with a scrambled or FDX2 siRNAs for 3 days, and the levels of FDX2, TAp73 and β-actin proteins were determined by western blotting. (H) The levels of FDX2, TAp73 and β-actin were measured in isogenic control and FDX2+/− HCT116 cells.