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. Author manuscript; available in PMC: 2020 Dec 18.
Published in final edited form as: J Pathol. 2020 May 18;251(3):284–296. doi: 10.1002/path.5451

Figure 4.

Figure 4.

Loss of FDXR reduces p73 transcript abundance. (A) The levels of FDXR, TAp73 and ACTB transcripts were examined by RT-PCR analysis in HCT116 cells transiently transfected with scrambled or FDXR siRNAs. (B) RKO cells were transiently transfected with scrambled or FDXR siRNAs for 3 days, followed by mock-treatment or treatment with doxorubicin for 18 h. The levels of FDXR, TAp73 and ACTB transcripts were examined by RT-PCR analysis. (C) Isogenic control and FDXR+/−/− HCT116 cells were mock-treated or treated with camptothecin or doxorubicin for 18 h, and the levels of FDXR, TAp73 and GAPDH transcripts were assessed by RT-PCR. (D) The levels of Fdxr, Trp73 and Actb transcripts were measured in two sets of WT and Fdxr+/− MEF. (E) The levels of FDX1, TAp73 and ACTB transcripts were measured in isogenic control and FDX1-KO HCT116 cells. (F) The levels of FDX2, TAp73 and ACTB transcripts were measured in isogenic control and FDX2+/− HCT116 cells.