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. 2020 Oct 24;7(6):619–625. doi: 10.1093/rb/rbaa041

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© The Author(s) 2020. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — The affected of GA concentration in different reaction systems. (A) S9 was inactivated by water bath heating. For inactive S9 group, CFU value was close to blank and showed a significant decrease compared with normal S9. (B) The green dotted line represents GA in S9 metabolic system; the red and blue full line represent GA in blank and inactive metabolic system, respectively. In S9 metabolic system, GA shows a rapid decline below the detection limit after at 30 min. For inactive S9 system, GA significantly decreases compared with blank. Due to the reaction system containing amino acids, which can crosslink with GA, over prolonged time GA concentration decreases. CFU, colony forming units. Mean ± SD, n = 3.