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. 2020 Dec 16;220(1):e202005165. doi: 10.1083/jcb.202005165

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© 2020 Feder et al.

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Figure 3. — The Sis1 interactome during heat shock. (A) Anti-FLAG IPs were performed from an untagged strain and a strain expressing Sis1-3xFLAG, both heat shocked for 15 min at 39°C. The significance over background is plotted as a function of the stoichiometry_app—the ratio of the background-subtracted quantitative value of each of the 192 interacting proteins to the value of Sis1. Proteins are color coded to match the categories in C. (B) Gene ontology associations enriched among the Sis1 interactors during heat shock. (C) Sis1 interactors grouped by functional category. Ribosomal proteins are abbreviated with their subunit designation (L or S) and their identifier. Proteins in gray are all those that do not belong to the other groups, enriched for highly expressed cytosolic enzymes. RNA pol II CTD, RNA polymerase II C-terminal domain.