Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Dec 16;220(1):e202005165. doi: 10.1083/jcb.202005165

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Feder et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 4. — Sis1 localizes to a perinucleolar ring and cytosolic clusters during heat shock. (A) Live-cell heat shock time course of cells expressing endogenously tagged Sis1-YFP and Hsp104-mKate. Cells were imaged by spinning disc confocal microscopy. Scale bar is 2 µm. (B) Cells expressing endogenously tagged Sis1-YFP and the nucleolar marker Cfi1-mKate imaged under nonstress and heat shock conditions. Scale bar is 2 µm. (C) Scans of fluorescence signal for Sis1-YFP (black) and Cfi1-mKate (purple) along the dashed lines in the merged images in B. (D) Cells expressing endogenously tagged Sis1-YFP and Hsp104-mKate following 10 min of heat shock after either no pretreatment or pretreatment with cycloheximide (CHX) to arrest protein synthesis. Scale bar is 2 µm. (E) Quantification of the fraction of cells with an Sis1 nuclear ring (black) and Hsp104 clusters (purple) after either no pretreatment or pretreatment with CHX. Experiments were performed with >50 cells in each replicate. Error bars represent the SD of the replicates (n = 3 for each strain and condition). (F) Live cells expressing endogenously tagged Sis1-mKate, Hsf1-YFP, and Hsp104-BFP were imaged in a lattice light-sheet microscope under nonstress conditions and following heat shock at 39°C for 15 min. MOC is a fraction of Sis1 that colocalizes with Hsf1. Scale bar is 2 µm. (G) Scans of fluorescence signal for Sis1-mKate (red) and Hsf1-YFP (green) along the dashed lines in the merged images in F.