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. 2020 Dec 16;220(1):e202005165. doi: 10.1083/jcb.202005165

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© 2020 Feder et al.

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Figure S4. — Sis1 perinucleolar localization is an early event following heat shock. (A) Images of fixed cells expressing Sis1-YFP and Hsp104-mKate over a series of early time points following heat shock. (B) Images of fixed cells expressing Sis1-YFP and Nsr1-mScarlet over a series of early time points following heat shock. Nsr1 is a nucleolar protein identified in the Sis1-3xFLAG IP/MS experiment (Fig. 3). Scale bar is 2 μm. (C) Quantification of the fraction of cells with Sis1 in nuclear rings and cytosolic clusters that colocalize with Hsf1. Experiments were performed in triplicate: Line shows the mean; box shows the SD; and error bars show the range.