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. 2020 Dec 16;220(1):e202005165. doi: 10.1083/jcb.202005165

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© 2020 Feder et al.

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Figure S5. — The proteostasis machinery forms a contiguous network on the ER. (A) Maximum-intensity negative projections of Sis1-YFP and Hsp104-BFP signal in cells under nonstress and heat shock conditions (39°C for 15 min). The signal is enhanced to show the cytosolic interconnections between the nodes. Scale bar is 2 µm. (B) The same as in A, but for the proteasome component Rpn1. (C) Overlay of Sis1-YFP and Rtn1-mScarlet from Fig. 5 C. Scale bar is 2 µm.