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. 2020 Dec 16;218(3):e20200547. doi: 10.1084/jem.20200547

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© 2020 Lu et al.

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Figure 3. — Adoptive transfer of regulatory CD4⁺ T cells rescues impairment in antigen-specific B cell responses. (A) Schematic for the Treg cell adoptive transfer experiment. (B) Left: Representative plots of HA-specific GC B cells as determined by HA probe in Bcl6^f/f, Bcl6^f/f Foxp3-Cre, and Bcl6^f/f Foxp3-Cre + Treg cell group. Right: Quantification of the percentages and numbers of HA-specific GC B cell responses with adoptive transfer strategy at day 38 p.i. (C) Left: Representative plot of the adoptively transferred Treg cell population defined as CD4⁺CD44^hiCD45.1⁺. Middle and right: Representative plots of the adoptively transferred population (CD45.1⁺) in the Tfh cell compartment defined as CD4⁺CD44^hiFoxp3⁻Ly6C⁻PSGL1^lowCXCR5^hiPD1^hi. (D) Representative plots of Tfr cells derived from the transferred Treg cells gated as CD45.1⁺ Foxp3⁺ in Bcl6^f/f, Bcl6^f/f Foxp3-Cre and Bcl6^f/f Foxp3-Cre + Treg cell animals. Tfr cells are defined as CD4⁺CD44^hiLy6C⁻PSGL1^loCXCR5^hiPD1^hiFoxp3⁺. Statistical analyses were performed using the unpaired two-tailed Student’s t test (*, P < 0.05; **, P < 0.01). Data are from one experiment representative of two experiments with four or five mice per group performed 38 d following influenza virus infection.