Fig. 1.

ABCB1/P-gp expression is decreased in Lrp1 knockout capillaries. (A) Representative immunoblots of ABCB1/P-gp in ex vivo capillaries isolated from control (Lrp1_BE^fl/fl) and brain endothelial-specific Lrp1 knockout (Lrp1_BE^−/−) mice. An anti-β-actin immunoblot is shown as a loading control. (B) Quantification of relative abundance of ABCB1/P-gp expression of n = 5 independent experiments. For each isolation ≥3 mice per group were used (mean ± SEM). For statistical analyses, unpaired t test was used. **P < 0.01.