Fig. 3.

Detection of MrNV and XSV by dot-blot hybridization assay developed in the present study. a Evaluation of detection threshold of the MrNV detection probes using total RNA extracted from viral filtrate (1:100 ng; 2:10 ng; 3:1 ng; 4:100 pg; 5:10 pg; 6:1 pg); b Evaluation of detection threshold of the XSV detection probes using total RNA extracted from viral filtrate (1:100 ng; 2:10 ng; 3:1 ng; 4:100 pg; 5:10 pg; 6:1 pg); c, d Dot hybridization using DIG-labelled probes for the experimentally infected samples; c MrNV, d XSV. *P: Positive; N: Negative