Fig. 4. CD47 regulates PMN migration across collagen-coated Transwell filters.

a Schematic representation of BMN migration in vitro in response to a chemoattractant gradient of LTB₄ (10 nM) for 2 hr at 37°C. b CD47 cell surface expression on BMN isolated from WT and Cd47^−/− mice by flow cytometry. c WT and Cd47^−/− BMN migration in response to LTB₄ in presence of blocking mAb against CD11b, CD47 or isotype control (20 μg/mL in each case). WT BMN migration was significantly reduced by addition of inhibitory CD11b or CD47 mAb. Loss of CD47 on Cd47^−/− BMN resulted in a significant reduced migration compared to WT BMN. Addition of blocking mAbs against CD11b or CD47 did not further reduce Cd47^−/− BMN migration in comparison to Cd47^−/− BMN migration in the presence of isotype mAb. Data are Means ± SEM of three independent experiments. *p ≤ 0.01 as determined by two-way ANOVA analysis.