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. Author manuscript; available in PMC: 2021 Mar 13.
Published in final edited form as: Mucosal Immunol. 2020 Jun 19;14(2):331–341. doi: 10.1038/s41385-020-0316-4

Fig. 6. CD47 regulates CD11b affinity for ligands.

Fig. 6.

a WT and Cd47−/− BMN express comparable surface levels of CD11a and CD11b integrins. b-d BMN were drawn across coverslips coated with immobilized ICAM-1-Fc, VCAM-1-Fc or E-selectin-Fc adhesion molecules at decreasing levels of shear stress ranging from 1.5 to 0.75 dynes/cm2, and BMN adhesion was measured as described in methods. In some experiments, BMN were pre-treated with function blocking mAbs anti-CD11a (M1/70; 20 μg/mL) or anti-CD11b (M1/70; 20 μg/mL). Data are Means ± SEM of at least three independent experiments. *p≤0.05, **p≤0.01, ***p≤0.001 as measured by ANOVA analysis followed by Tukey’s multiple comparison test.