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. Author manuscript; available in PMC: 2021 Dec 10.
Published in final edited form as: J Control Release. 2020 Sep 30;328:710–721. doi: 10.1016/j.jconrel.2020.09.048

Table 2.

In vitro study design to study the combined effect of the presence of PLL and varying coculture ratios of MSCs and ACs on chondrogenic and hypertrophic gene expression.

Group (n = 4) MSC-to-AC Ratio PLL MW (kDa) PLL concentration (µg/ml) Total cell concentration
MSC AC
MSC 1 0
3:1 3 1
1:1 1 1 0, 225 kDa 0, 50 µg/ml 30 million cells/ml
1:3 1 3
AC 0 1

Hydrogel volume: 15 µl (3 mm diameter × 2 mm height molds)