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. Author manuscript; available in PMC: 2020 Dec 19.
Published in final edited form as: ACS Synth Biol. 2020 Nov 12;9(12):3322–3333. doi: 10.1021/acssynbio.0c00397

Figure 7.

Figure 7.

Light-switchable protein translocation. a) Schematic of the light-induced reversible binding of cytoplasmic LDB-3-mCherry to membrane-bound DrBphP-AcGFP. b) Fluorescence images of a HEK293T cell coexpressing LDB-3-mCherry (red) and DrBphP-AcGFP (green) for 24 h and then subjected to three illumination steps: i) 775 nm (0.2 mW/cm2, 10 min; left), ii) 654 nm (0.2 mW/cm2, 2 min; middle), and iii) 775 nm (0.2 mW/cm2, 10 min; right). Bars, 10 μm. c) Intensity profile of mCherry fluorescence intensities along white dashed lines in b). 5 μM biliverdin was supplemented in the medium.