Table 1.

Primer sequences and location.

	Primer	Sequence		Tm (°C)	GC (%)	PCR product size	Location with respect to TSS	Location with respect to ATG
ESR2 promoter 0N	→PCR	GGTATTTTTTAGGATTTGGTTGGAAATGTA	30	60,9	30,0	276 bp	− 239	− 11,663
	←PCRB	ACTTAACCATAAACCCCTTCTTCCTTT	27	58,9	37,0		+ 37	− 11,387
	SEQ	ATATTTTTAGGTTTTATTTTAGAT	24	40,7	12,5	–	− 209	− 11,633
ESR2 exon 0N	→PCR	GGAGGTTGAGAGAAATAATTGTTTTTTGA	29	57,7	31,0	253 bp	+ 115	− 11,309
	←PCRB	AAACACACCCACCTTACCTTCTCTA	25	58,3	44,0		+ 368	− 11,057
	SEQ	GTTTTTTGAAATTTGTAGGG	20	44,8	30,0	–	+ 135	− 11,289

 →PCR, forward primer;  ←PCR, reverse primer; ^B, biotinylated primer; Tm, melting temperature, GC, guanine-cytosine content; bp, base pairs; TSS, transcription start site; ATG, start codon; SEQ, sequencing primer.