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. 2020 Dec 18;10:22294. doi: 10.1038/s41598-020-79448-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Graphical representation of the experimental plan. (a) Cell culture processing of tumour parental cells and sarcospheres of both cell lines for the development of the 3D scaffold-based models of osteosarcoma. (b) Sarcospheres characterization of both cell lines compared to 2D parental cells. (c) Experimental plan and characterization of the two phenotypes (parental and sarcospheres) in the 3D osteosarcoma models. Scaffold-free sarcospheres were used as a control.