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. 2020 Dec 18;10:22289. doi: 10.1038/s41598-020-79407-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Antigen-specific cytokine responses in mice vaccinated with PBS, Silirum, recombinant proteins (Rec-protein), control protein particles (Control), and MAP fusion protein particles (MAP-fusion). Cells were isolated from AXLN and SPLN of the vaccinated mice and stimulated in vitro with media alone or a mixture of 33 peptides covering Ag85A, SOD, Ag85B, and 74F at a final concentration of 10 µg/mL for 72 h. Cell culture supernatants from AXLN and SPLN were analysed for IFN-γ; IL-17A; and IL-10 production by ELISA. *Significantly different to the PBS group (P < 0.05).