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. 2020 Oct 31;20:86–94. doi: 10.1016/j.omtm.2020.10.022

Figure 2.

Figure 2

Summary of relative fold gene expression of TJ proteins, IOP, and facility changes after intracameral siRNA injection in vehicle control animals

(A) A mean fold change in relative gene expression of 0.7 [0.5, 0.8] (p < 0.001, one-sample t test to theoretical mean of 1, n = 12) and 0.6 [0.4, 0.9] (p < 0.01, one-sample t test to theoretical mean of 1, n = 11) is shown in both ZO-1 and tricellulin protein transcripts, respectively. Error bars represent minimum/maximum values, the 95% CI is shown by bounds of box, and the horizontal line represents the median. (B) Cello plots showing the change in IOP (mmHg) 48 h post-treatment with either non-targeting siRNA (NT-siRNA) (orange) or siRNA targeting ZO-1 and tricellulin (T-siRNA) (green). The white line represents the geometric mean, the dark orange/green bands indicate the 95% CI, and the light orange/green regions are the distributions of the data. Each data point represents the change in IOP (mmHg) for each individual animal. (C) Summary of changes in IOP and outflow facility after treatment with intracameral injection of T-siRNA (green, n = 9) or NT-siRNA (orange, n = 9) in vehicle-treated mice. Differences in ex vivo outflow facility (C) 72 h after treatment are shown via green arrows. Dashed arrows represent the differences between population average pre-treatment and 48 h post-treatment, while solid arrows represent the average change in IOP per mouse after treatment. (D) Intracameral injection of T-siRNA targeting ZO-1 and tricellulin is shown to significantly increase conventional outflow facility in treated eyes as compared to contralateral control eyes receiving NT-siRNA in vehicle normotensive animals, with an increase of 38 [5, 81] % (mean [95% CI]) (p = 0.029, n = 6 pairs). The white line represents the geometric mean, the dark blue bands indicate the 95% CI, and the light blue regions are the distribution of the data. Each data point represents the difference in C between contralateral eyes with 95% CI. p values are represented by asterisks.