Figure 3.

Summary of relative fold gene expression of TJ proteins, IOP, and facility changes after intracameral siRNA injection in DEX-treated animals

(A) A mean fold change in relative gene expression of 0.6 [0.2, 1.0] (p < 0.05, one-sample t test to theoretical mean of 1, n = 8) and 0.4 [0.1, 0.6] (p < 0.01, one sample t test to theoretical mean of 1, n = 6) was shown in both ZO-1 and tricellulin protein transcripts, respectively. Error bars represent minimum/maximum values, the 95% CI is shown by bounds of box, and the horizontal line represents the median. (B) Cello plots showing the change in IOP (mmHg) 48 h post-treatment with either NT-siRNA (orange) or T-siRNA (green). The white line represents the geometric mean, the dark orange/green bands indicate the 95% CI, and the light orange/green regions are the distributions of the data. Each data point represents the change in IOP (mmHg) for each individual animal. (C) Summary of changes in IOP and outflow facility after treatment with intracameral injection of siRNA targeting ZO-1 and tricellulin (T-siRNA, green, n = 17) or NT-siRNA (orange, n = 17) in DEX-treated mice. (D) These cello plots show the significant increase in conventional outflow facility following treatment with T-siRNA compared to contralateral control eyes receiving NT-siRNA in DEX-treated animals, with an increase of 63 [20, 122] % (mean [95% CI]) (p = 0.0071, n = 8 pairs). The white line represents the geometric mean, the dark blue bands indicate the 95% CI, and the light blue regions are the distribution of the data. Each data point represents the difference in C between contralateral eyes with 95% CI. p values are represented by asterisks.