Fig. 3.
In vivo biodistribution studies of three F8-4-1BBL formats. (A) The mice were euthanized at 24 h after injection of the radioiodinated proteins, and the radioactivity of excised organs was measured and expressed as percent injected dose per gram of tissue (%ID/g ± SD n = 3). The KSF antibody targeting hen egg lysozyme served as an untargeted control (67). (B) The mice were euthanized at 24 h after the injection of FITC-labeled F8-4-1-BBL or KSF-4-1-BBL in format 5. The proteins were detected ex vivo on cryosections (green, αFITC; red, αCD31).