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. 2020 Dec 2;117(50):31789–31799. doi: 10.1073/pnas.2013968117

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Fig. 6. — Use of the peroxisomal GPP-synthesizing microfactory for the production of CBGA. The geranyl transferase CsPT4 from C. sativa and the GPP synthase Erg20p(N127W) from S. cerevisiae were introduced into yeast cells producing GPP from the engineered peroxisomal pathway, to give strain PERCan02. Conversion of OA into CBGA is observed in strain PERCan02 compared to the control strains lacking CsPT4 and Erg20p(N127W) (strain PERMva01) or lacking the peroxisomal GPP-producing pathway (strain PERCan01). OA and CBGA presence in the extracts was assessed by LC–electrospray ionization–MS analysis and extracted ion chromatograms for the mass of OA (223.0982 ± 0.02 g/mol) and CBGA (359.2226 ± 0.02 g/mol) are presented here.