Fig. 7.

Fed-batch culture production of geraniol and (R)-(+)-limonene by harnessing the engineered peroxisomal monoterpene pathway. Semicontinuous fed batch flask cultures of strains PERGer02 and PERLim19 were conducted to evaluate the maximal monoterpene production of the peroxisomal pathway under high cell-density conditions. In both cases, cultures could be sustained over a prolonged period of time allowing for a continuous and steady production of the corresponding monoterpene. (A) Cell optical density and geraniol production by strain PERGer02. (B) Cell optical density and limonene production by strain PERLim19. Cultures were fed with galactose/raffinose and the pH adjusted back to 4.5 every 48 h. Error bars correspond to the SD around the mean (n = 3, corresponding to three biological replicates).