Evaluation of recurrent adhesion prevention on days 7 and 14 after the second surgery in a rat repeated-injury model. (A) Severe adhesions were observed in the control and Interceed film groups while no adhesion was observed in rats treated with PCBAA on days 7 and 14 after the second surgery. (B and C) Distribution of adhesion scores (B) and weight loss (as percentage of starting body mass) (C) in the untreated control, film, and PCBAA groups on days 7 and 14 after the second surgery. The results are presented as mean ± SD (n = 6). **P < 0.01; NS, not significant. (D) Representative histology images (H&E and Masson trichrome staining) of tissues from different groups on days 7 and 14 after the second surgery, respectively. AW: abdominal wall; CE: cecal mucosa; Me: mesothelial layer; SK: skeletal muscle of AW. The deposited collagen in the adhesion site was stained in blue while muscle showed red in Masson trichrome staining. (Scale bar, 400 μm.)