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. 2020 Nov 25;117(50):32017–32028. doi: 10.1073/pnas.2016451117

Fig. 3.

Fig. 3.

NLPs loaded with MOG35–55 and the tolerogenic AhR ligand ITE. (A) Schematic representation of NLPITE+MOG. (B) NLP characterization, including size, polydispersity index (PDI), zeta potential, % ITE, and % MOG35–55 encapsulation. Values represent the average of at least 17 different batches ± SD. (C) mRNA expression of AhR target genes cyp1a1 and cyp1b1 in murine splenic DCs following incubation for 6 h with NLP, NLPMOG, NLPITE (containing 10 nM ITE), NLPITE+MOG (containing 10 nM ITE), or free ITE (10 nM). (D) Proliferative response of 2D2 transgenic splenocytes activated for 72 h with NLP, NLPMOG (containing 10 μg/mL MOG), NLPITE (containing 1 μg/mL ITE), NLPITE+MOG (containing 10 μg/mL MOG and 1 μg/mL ITE), or free MOG (10 μg/mL). Data from C and D are means + SEM of one experiment representative of at least three independent experiments, with at least three biological replicates per experiment. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, one-way ANOVA followed by Dunnett’s multiple comparison test.