Fig. 6.

NLP_ITE+MOG suppresses the encephalitogenic T cell response. (A) Proliferative response and (B) cytokine secretion following restimulation with MOG_35–55 of splenocytes from SC NLP-treated B6 mice, 26 d after EAE induction. (C) Gating strategy for the identification of MOG_38–49 tetramer-positive IL-17⁺, IFNγ⁺, FoxP3⁺, and IL-10⁺ CD4⁺ T cells. (D) Frequency of MOG_38–49 tetramer-positive IL-17⁺, IFNγ⁺, FoxP3⁺, and IL-10⁺ CD4⁺ T cells in the brain and spinal cord of B6 EAE SC NLP-treated mice. Data are mean + SEM (n = 5 mice per group). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, one-way ANOVA followed by Dunnett’s multiple comparison test.