Figure 2.

PDA Produced and Deposited on Cell Membrane

(A) Flow cytometry histogram of CCRF-CEM cells treated with HEPES buffer (cell only group), Chol-AS1411, hemin, and EGFP (“control” group), only enhanced green fluorescent protein (“GFP” group), control + K⁺ (“K⁺ + GFP” group), control + H₂O₂ (“H₂O₂+ GFP” group), and control + K⁺ + H₂O₂ (“H₂O₂+ K⁺ + GFP” group). Results show that EGFP stained the deposited PDA on the cell membrane but only in the presence of K⁺ and H₂O₂. EGFP concentration = 500 nM.

(B) Confocal microscopy images of CCRF-CEM cells treated with different reagents to produce PDA; EGFP concentration = 2 μM; scale bar: 50 μm.