Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Dec 16;25:100871. doi: 10.1016/j.bbrep.2020.100871

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Fig. 2 — Measurement of the interaction between P9-EP4 and various G protein alpha subunits by ELISA. (A) The purified G_αi1 (i1), G_αi2 (i2), G_αi3 (i3), and G_αs1 (s1) were analyzed by SDS-PAGE followed by Coomassie staining. (B, C) The concentration-dependent binding of P9-EP4 in sarkosyl (B) or in APG (C) to the immobilized G_α proteins. Five to 1000 nM of P9-EP4 was applied to the plate treated with 5% of skimmed milk blocking solution (filled circle), or to the plate immobilized with 10 μg/ml of G_αi1 (open circle), G_αi2 (filled triangle), G_αi3 (filled square), or G_αs1 (filled diamond), and the amount of bound P9-EP4 was measured using an anti-P9 antibody.