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. 2020 Jun 19;15(12):2296–2305. doi: 10.4103/1673-5374.285006

Figure 1.

Figure 1

ZL006 protects against Aβ1–42-induced neuronal cell death.

(A) The chemical structure of ZL006. (B) Primary cortical neurons at DIV 10–12 were treated with ZL006 (0–150 μM) or DMSO vehicle (< 1‰) for 24 hours and CCK8 assay was performed. n = 8–11 cells per group. (C) Primary cortical neurons at DIV 10–12 were pretreated with ZL006 (0–150 μM) or DMSO vehicle (< 1‰) for 2 hours and then treated with Aβ1–42 (2 μM) or NH3•H2O vehicle (< 1‰) for 24 hours, and cell viability was accessed. n = 6 cells per group. (D) Primary cortical neurons at DIV 10–12 were pretreated with ZL006 (75 μM) or DMSO vehicle (< 1‰) for 2 hours and then treated with Aβ1–42 (2 μM) or NH3•H2O vehicle (< 1‰) for 24 hours, and calcein-acetoxymethylester/ PI staining was performed. Representative images of neurons stained by calcein-AM/PI were shown. Original magnification, 20×, scale bar: 50 μm. (E) Quantitative analysis of the PI+ cells, n = 6 cells per group. Data were expressed as mean ± SEM (oneway analysis of variance followed by Bonferroni's post hoc test). *P < 0.05, **P < 0.01, vs. NH3•H2O vehicle group; #P < 0.05, ##P < 0.01, vs. Aβ1–42 + DMSO vehicle group. Aβ: Amyloid-beta; AM: acetoxymethylester; DIV: day- in-vitro; DMSO: dimethyl sulfoxide; PI: propidium iodide.