Figure 2.

ZL006 ameliorates Aβ_1–42-induced apoptosis in primary neurons.

Primary neurons at DIV 10–12 were pretreated with ZL006 (75 μM) or DMSO vehicle (< 1‰) for 2 hours and then treated with Aβ_1–42 (2 μM) or NH₃•H₂O vehicle (< 1‰) for 24 hours, and Annexin V-FITC staining analysis was performed. Original magnification, 40×, scale bar: 20 μm. (B) Quantitative analysis of green fluorescence intensity. n = 3 cells per group. (C) The level of cleaved caspase-3 and MAP2 of primary cortical neurons at DIV 11–13 was determined by immunostaining. Original magnification, 20×, scale bar: 50 μm. (D) Quantitative analysis of the level of cleaved caspase-3. n = 4 cells per group. (E) The protein expressions of Bax and Bcl-2 in primary neurons at DIV 11–13 were examined by western blot assay. n = 4 cells per group. Data were expressed as mean ± SEM (one-way analysis of variance followed by Bonferroni's post hoc test). *P < 0.05, **P < 0.01, vs. NH₃•H₂O group; #P < 0.05, ##P < 0.01, vs. Aβ_1–42 + DMSO group. Aβ: Amyloid-beta; DAPI: 4',6-diamidino-2-phenylindole; DIV: day- in-vitro; DMSO: dimethyl sulfoxide; FITC: fluoresceine isothiocyanate; MAP2: microtubule-associated protein 2.