Figure 2.

Peripheral nerve injury alters forkhead class box O3a (FOXO3a) immunoreactivity in dorsal root ganglion (DRG) neurons ipsilateral to axotomy.

(A) Immunofluorescence photomicrographs of L5 DRG sections (6 μm) processed to detect FOXO3a immunoreactivity. Ipsilateral DRG from L4–6 spinal nerve transected rats were collected after the predetermined time points 1 hour, 1 day, 2 days, 4 days and 1 week post-injury. Scale bar: 50 μm. Naïve animals served as controls. (B) Representative scatterplots depicting relative changes in FOXO3a immunofluorescence signal over individual cytoplasmic and nuclear regions as related to cell size from sections all mounted on the same slide and represented in photomicrographs, thus all processed under identical conditions. Experimental states as indicated. Dashed lines divide the plots into low versus moderately to heavily labelled populations. n = 217 to 294 neurons analyzed per condition.