Skip to main content
. Author manuscript; available in PMC: 2020 Dec 19.
Published in final edited form as: Clin Sci (Lond). 2020 Jul 17;134(13):1751–1762. doi: 10.1042/CS20200575

Figure 4. Neutralizing HMGB1 reduced renal IR-induced neutrophil infiltration 24 h post-IR only in male SHR.

Figure 4.

Renal neutrophil infiltration was assessed by in a blinded manner by immunohistochemical analysis of myeloperoxidase staining in kidney samples collected 24 h post-IR in 13 week old male and female SHR treated with control IgG or HMGB1 neutralizing antibody 1 h prior to IR. Brown nuclei staining indicating neutrophil positive cells. Representative images are provided in (A) with the quantification in (B). Renal chemokines and receptor mRNA expression were measured by RT-PCR (C–G). Data are expressed as means ± SEM, n=5–6 rats in each group with individual animal data indicated by the symbols. Open symbols indicate control IgG-treated animals, filled symbols indicate anti-HMGB1-treated animals, males are represented by squares and females by circles. Data were compared via two-way ANOVA with P<0.05 considered significant.