Skip to main content
. Author manuscript; available in PMC: 2020 Dec 19.
Published in final edited form as: Clin Sci (Lond). 2020 Jul 17;134(13):1751–1762. doi: 10.1042/CS20200575

Figure 7. Neutralization of HMGB1 attenuates tubular injury in male SHR compared with females 24 h post-IR.

Figure 7.

Tubular injury was assessed via histological analysis and measurement of renal KIM-1 mRNA expression via RT-PCR in kidneys collected 24 h post-IR in 13 week old male and female SHR treated with control IgG or HMGB1 neutralizing antibody 1 h prior to IR. Representative images of tubular damage are shown in (A) (10× magnification, scale bar 200 μM) with the mean pathological scoring data in (B). (C) is the renal KIM-1 mRNA expression normalized to GAPDH. Data are expressed as mean ± SEM, n=5–6 rats in each group with individual animal data indicated by the symbols. Open symbols indicate control IgG-treated animals, filled symbols indicate anti-HMGB1-treated animals, males are represented by squares and females by circles. Data were compared via two-way ANOVA with P<0.05 considered significant.