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. 2020 Sep 2;11(6):1705–1722. doi: 10.1002/jcsm.12617

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© 2020 The Authors. Journal of Cachexia, Sarcopenia and Muscle published by John Wiley & Sons Ltd on behalf of Society on Sarcopenia, Cachexia and Wasting Disorders

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Gastrocnemius myonuclear analysis with training and detraining. (A–D) Representative images of longitudinal single muscle fibres from 6M UT, 6M PoW, 12M UT, and 12M PoW + DT. (E) Single fibre analysis of myonuclear number. (F) Immunohistochemistry (IHC) analysis of myonuclear number. (G) Fibre type‐specific myonuclear number via IHC. (H) Myonuclear length. *6M UT versus 6M PoW, ^6M UT versus 12M UT, ^#12M DT versus 12M PoW + DT, ^†6M PoW versus 12M PoW + DT. All P < 0.05, scale bars = 50 μm, n ≥ 7 per group for each analysis.