Figure 5.

Mitochondrial function in soleus muscle. (A) Citrate synthase activity and the activity of complex I, complex II, complex III, and complex IV of the respiratory chain were measured in soleus muscle lysates. All values were normalized to total protein content of the samples. (B) Active rates of respiration (state 3) were measured in saponin‐skinned fibres of soleus muscle in the presence of 5 mM ADP and either 10 mM pyruvate + 2 mM malate or 10 mM succinate in the presence of 5 μM rotenone. All data are mean ± standard error of the mean. n = 6 samples per group. ^* P < 0.05. AOB, ascending aortic banding; PAB, pulmonary artery banding.